shake flask method procedure

This can be done using a syringe with a removable needle. Log D 7.4 shake flask is one of Cyprotex's in vitro ADME screening services. Now to your questions: * How much do you “shake” the flask? This commonly used shake flask protocol is based on the use of Millipore Multiscreen solubility filter plates or centrifugation followed by UV-Vis quantitation of dissolved compounds. Shake flask solubility determinations are quite straightforward but there are a few mistakes that are easy to make for a beginner. Service details. Use the Log D shake flask assay to determine the lipophilicity of your compound. Note: Make sure that the shaker flask does not have baffles (i.e., the indents at the bottom of the flask designed to provide agitation), because they ruin the shaking rhythm. Measurement of Log D by the shake flask method as a measure of lipophilicity Shake flask-type. The aqueous phase should be sampled by a procedure that minimizes the risk of including traces of n-octanol. shake flask experiment and some of these alternatives have been recently reviewed, and the shake flask derived data are still viewed as the gold standard in terms of quality but with signifi-cant drawbacks in terms of sample throughput capacity.12 the method that has been developed is a miniaturized and automated shake flask log D 7.4 Several procedures based on the shake-flask method and designed to require a minimum amount of drug for octanol-water partition coefficient determination have been established and developed. Several procedures based on the shake-flask method and designed to require a minimum amount of drug for octanol-water partition coefficient determination have been established and developed. Cyprotex deliver consistent, high quality data with cost-efficiency that comes from a highly automated approach. The procedures have been validated by a 28 substance set with a lipophilicity range from -2.0 to 4.5 (logD7.4 … The classical and most reliable method of log P determination is the shake-flask method, which consists of dissolving some of the solute in question in a volume of octanol and water, then measuring the concentration of the solute in each solvent. Measurements are usually performed by the shakeflask method with UV-Vis or LC-MS detection. A new method for determination of distribution coefficient of drugs azathioprine, 6-mercaptopurine and 6-thioguanine and nutrient folic acid used in the treatment of inflammatory bowel disease based on a miniaturized shake-flask and HPLC/DAD was developed. Abstract. When the cells are ready for passaging (i.e., log-phase growth before they reach confluency), remove the flask from the incubator and take a small sample from the culture flask using a sterile pipette.

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